HNRNPF anticorps

N° du produit ABIN272205

Cet anticorps anti-HNRNPF Polyclonal Lapin (ABIN272205) détecte spécifiquement HNRNPF dans WB et IF. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour HNRNPF anticorps (ABIN272205)

Antigène: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HNRNPF est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-HNRNPF anticorps

Specificité: This antibody detects endogenous levels of hnRNP F protein. (region surrounding Tyr47)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Information d'application

Indications d'application: ELISA: 1/5000approx. 1/10000. Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur HNRNPF

Antigène: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Autre désignation: HnRNP-F / HNRNPF

Sujet: Heterogeneous Nuclear Ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP complexes are the major constituents of the spliceosome and in particular, the hnRNP A1 protein is one of the major premRNA/mRNA binding proteins and also one of the most abundant proteins in the nucleus. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The majority of hnRNP proteins components are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, including hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA.Synonyms: HNRPF, Heterogeneous nuclear ribonucleoprotein F, Nucleolin-like protein mcs94-1

Poids moléculaire: approx. 48 kDa

ID gène: 3185

NCBI Accession: NP_001091674

UniProt: P52597