SMAD4 anticorps

N° du produit ABIN272224

Cet anticorps Lapin Polyclonal détecte spécifiquement SMAD4 dans WB, IF et IHC (p). Il présente une réactivité avec des échantillons de Humain, Souris et Rat. Il a été cité dans 6+ publications.

Aperçu rapide pour SMAD4 anticorps (ABIN272224)

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SMAD4 anticorps

Specificité: This antibody detects endogenous levels of Smad4 protein. (region surrounding Leu43)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Références pour anticorps anti-SMAD4 (ABIN272224)

Nagappan, Park, Park, Kim, Hong, Kang, Zhang, Kim, Lee, Won, Kim: "Proteomic analysis of differentially expressed proteins in vitamin C-treated AGS cells." dans: BMC biochemistry, Vol. 14, pp. 24, (2014) (PubMed).

Yang, Zhao, Rasul, Qin, Li, Li: "PIAS1-modulated Smad2/4 complex activation is involved in zinc-induced cancer cell apoptosis." dans: Cell death & disease, Vol. 4, pp. e811, (2013) (PubMed).

Yang, Li, Wang, Wang, Li, Yang, Peng: "Smad4 disruption accelerates keratinocyte reepithelialization in murine cutaneous wound repair." dans: Histochemistry and cell biology, Vol. 138, Issue 4, pp. 573-82, (2012) (PubMed).

Yan, Zhang, Fang, Zhang, Wu, Jiang, Sun, Hu: "MicroRNA-145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB." dans: FEBS letters, Vol. 586, Issue 19, pp. 3263-70, (2012) (PubMed).

Izumi, Nakamura, Tokumo, Mano: "A minute pancreatic ductal adenocarcinoma with lipomatous pseudohypertrophy of the pancreas." dans: JOP : Journal of the pancreas, Vol. 12, Issue 5, pp. 464-8, (2011) (PubMed).

Yuan, Wang, Hu, Li, Jing: "Transforming growth factor-?/Smad signaling function in the aortopathies." dans: Revista brasileira de cirurgia cardiovascular : órgão oficial da Sociedade Brasileira de Cirurgia Cardiovascular, Vol. 26, Issue 3, pp. 393-403, (2011) (PubMed).

Détails sur SMAD4

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Autre désignation: SMAD4

Sujet: Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.Synonyms: DPC4, Deletion target in pancreatic carcinoma 4, MAD homolog 4, MADH4, Mothers against DPP homolog 4, Mothers against decapentaplegic homolog 4, SMAD 4, SMAD family member 4, SMAD-4

Poids moléculaire: approx. 60 kDa

ID gène: 4089

NCBI Accession: NP_005350

UniProt: Q13485

Pathways: Cycle Cellulaire, Chromatin Binding, Autophagy