Cyclin A anticorps

N° du produit ABIN2740254

L’anticorps Souris Monoclonal anti-Cyclin A a été validé pour WB, IHC, IF, ELISA, FACS, IP et IHC (p). Il convient pour détecter Cyclin A dans des échantillons de Humain, Souris, Boeuf (Vache), Green Monkey et Vison.

Aperçu rapide pour Cyclin A anticorps (ABIN2740254)

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivité: Humain, Souris, Boeuf (Vache), Green Monkey, Vison

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: E67

Détail du produit anti-Cyclin A anticorps

Specificité: Bovine Cyclin A1+2

Purification: Protein G purified

Immunogène: Full length bovine protein.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Information d'application

Indications d'application: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (1 - 2 μg/mL), IHC, IHC-P (1 - 2 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: This antibody recognizes a protein of 54  kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase. ELISA: Order BSA-free antibody format for coating.

Commentaires:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2°C to 8°C.

Détails sur Cyclin A

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Autre désignation: Cyclin A

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA