NGFR anticorps (Biotin)
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- Antigène Voir toutes NGFR Anticorps
- NGFR (Nerve Growth Factor Receptor (NGFR))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp NGFR est conjugé à/à la Biotin
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Application
- Flow Cytometry (FACS), ELISA
- Specificité
- Antibody ANC271/3D7 binds to recombinant CD271-muIg in EIA, and to endogenous CD271 on cell surface of human Reh cells.
- Purification
- Protein A purified
- Immunogène
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Recombinant soluble human CD271.
Type of Immunogen: Recombinant protein - Clone
- ANC271-3D7
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product NGFR Anticorps primaire
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- Indications d'application
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Approved: ELISA, Flo
Usage: Five x 105 cultured human SW480 cells were harvested by EDTA, washed and pre incubated with 20 μL of 300 μg/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-CD271/Biotin at a concentration of 10 μg/mL. Cells were then washed twice and incubated with secondary reagent Streptaviden/R-PE. They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.17 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD271 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Store at 4°C, avoid freeze-thaw cycles.
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- Antigène
- NGFR (Nerve Growth Factor Receptor (NGFR))
- Autre désignation
- NGFR / CD271 (NGFR Produits)
- Synonymes
- anticorps ngfr, anticorps wu:fb38c02, anticorps wu:fc10h01, anticorps CD271, anticorps Gp80-LNGFR, anticorps TNFRSF16, anticorps p75(NTR), anticorps p75NTR, anticorps LNGFR, anticorps RNNGFRR, anticorps Tnfrsf16, anticorps p75, anticorps NRH1b, anticorps fullback, anticorps nrh1, anticorps p75ntr, anticorps tnfrsf16, anticorps p75NGFR, anticorps nerve growth factor receptor, anticorps neurotrophin receptor associated death domain, anticorps nerve growth factor receptor S homeolog, anticorps nerve growth factor receptor (TNFR superfamily, member 16), anticorps NGFR, anticorps nradd, anticorps Ngfr, anticorps ngfr.S
- Sujet
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Name/Gene ID: NGFR
Family: TNF Receptor
Synonyms: NGFR, CD271, CD271 antigen, gp80-LNGFR, Nerve growth factor receptor, p75 ICD, p75(NTR), p75NTR, TNFR superfamily, member 16, NGF receptor, TNFRSF16 - ID gène
- 4804
- Pathways
- Signalisation NF-kappaB, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding
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