Poliovirus Receptor anticorps (Biotin)

N° du produit ABIN2743906

Cet anticorps Souris Monoclonal détecte spécifiquement Poliovirus Receptor dans ELISA et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour Poliovirus Receptor anticorps (Biotin) (ABIN2743906)

Antigène: Poliovirus Receptor (PVR)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Poliovirus Receptor est conjugé à/à la Biotin

Application: ELISA, Flow Cytometry (FACS)

Clone: ANC2B2

Détail du produit anti-Poliovirus Receptor anticorps (Biotin)

Specificité: Antibody ANC2B2 binds to cell surface CD155 on U-937 cells, and binds to recombinant CD155 in EIA.

Purification: Protein A purified

Immunogène: Recombinant Human CD155.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA, Flo

Usage: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 μg/mL PHA. Five x 105 cultured human U-937 cells per tube were washed and pre incubated 5 minutes with 20 μL of 300 ^g/mL human IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-human CD155/Biotin at concentration of 10 ^g/mL. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.8 log10 fluorescent units when compared to an Isotype control Mouse IgG1/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD155-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 4°C, avoid freeze-thaw cycles.

Détails sur Poliovirus Receptor

Antigène: Poliovirus Receptor (PVR)

Autre désignation: PVR / CD155

Sujet: Name/Gene ID: PVR

Synonyms: PVR, CD155, Hpvr, HVED, Nectin-like 5, Nectin-like protein 5, Poliovirus receptors beta, Poliovirus receptor, PVS, Necl-5, CD155 antigen, NECL5, TAGE4

ID gène: 5817

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome