MICA anticorps

N° du produit ABIN2749085

Cet anticorps Souris Monoclonal détecte spécifiquement MICA dans ELISA, FACS, IP, ICC, IHC (fro) et MS. Il présente une réactivité avec des échantillons de Humain, Boeuf (Vache), Cat et Non-Human Primate. Il a été cité dans 9+ publications.

Aperçu rapide pour MICA anticorps (ABIN2749085)

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Reactivité: Humain, Boeuf (Vache), Cat, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MICA est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Mass Spectrometry (MS)

Clone: W6-32

Détail du produit anti-MICA anticorps

Fonction: Anti-HLA Class I Purified

Specificité: The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.

Aucune reactivité croisée: Lapin

Réactivité croisée (Details): Human, Non-Human Primates, Bovine, Feline (Cat)

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Membrane of human tonsil cells

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-12 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-MICA (ABIN2749085)

Le Discorde, Moreau, Sabatier, Legeais, Carosella: "Expression of HLA-G in human cornea, an immune-privileged tissue." dans: Human immunology, Vol. 64, Issue 11, pp. 1039-44, (2003) (PubMed).

Tran, Ivanyi, Hilgert, Brdicka, Pla, Breur, Flieger, Ivasková, Horejsí: "The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex." dans: Immunogenetics, Vol. 53, Issue 6, pp. 440-6, (2001) (PubMed).

Ladasky, Shum, Canavez, Seuánez, Parham: "Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody." dans: Immunogenetics, Vol. 49, Issue 4, pp. 312-20, (1999) (PubMed).

Shields, Ribaudo: "Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin." dans: Tissue antigens, Vol. 51, Issue 5, pp. 567-70, (1998) (PubMed).

Jacobsen, Aasted, Broe, Petersen: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." dans: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, (1994) (PubMed).

Stern, Beresford, Friedman, Stevens, Risk, Johnson: "Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 4, pp. 1088-91, (1987) (PubMed).

Neefjes, Hensen, de Kroon, Ploegh: "A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes." dans: Immunogenetics, Vol. 23, Issue 5, pp. 341-7, (1986) (PubMed).

Brodsky, Parham: "Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies." dans: Immunogenetics, Vol. 15, Issue 2, pp. 151-66, (1982) (PubMed).

Barnstable, Bodmer, Brown, Galfre, Milstein, Williams, Ziegler: "Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis." dans: Cell, Vol. 14, Issue 1, pp. 9-20, (1978) (PubMed).

Détails sur MICA

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Autre désignation: HLA Class I

Sujet: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response