DDX21 anticorps (N-Term)

N° du produit ABIN2775197

L’anticorps Lapin Polyclonal anti-DDX21 a été validé pour WB. Il convient pour détecter DDX21 dans des échantillons de Humain, Rat, Cheval et Saccharomyces cerevisiae. Il y a 2+ publications disponibles.

Aperçu rapide pour DDX21 anticorps (N-Term) (ABIN2775197)

Antigène: DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21))

Reactivité: Humain, Rat, Cheval, Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DDX21 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-DDX21 anticorps

Épitope: N-Term

Séquence: MPGKLRSDAG LESDTAMKKG ETLRKQTEEK EKKEKPKSDK TEEIAEEEET

Homologie: Horse: 79%, Human: 100%, Rat: 91%, Yeast: 83%

Attributs du produit: This is a rabbit polyclonal antibody against DDX21. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human DDX21

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 783 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-DDX21 (ABIN2775197)

De Wever, Lloyd, Nasa, Nimick, Trinkle-Mulcahy, Gourlay, Morrice, Moorhead: "Isolation of human mitotic protein phosphatase complexes: identification of a complex between protein phosphatase 1 and the RNA helicase Ddx21." dans: PLoS ONE, Vol. 7, Issue 6, pp. e39510, (2012) (PubMed).

Naji, Ambrus, Cimerman?i?, Reyes, Johnson, Filbrandt, Huber, Vesely, Krogan, Yates, Saphire, Gerace: "Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production." dans: Molecular & cellular proteomics : MCP, Vol. 11, Issue 4, pp. M111.015313, (2012) (PubMed).

Détails sur DDX21

Antigène: DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21))

Autre désignation: DDX21

Sujet: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription.
Alias Symbols: GUA, GURDB, RH-II/GU, RH-II/GuA
Protein Interaction Partner: HUWE1, PA2G4, KPNA3, CEP250, SUMO2, SUMO3, STAU1, IVNS1ABP, UBC, MDM2, LGR4, SUMO1, ERG, WWOX, EED, rev, SRPK2, SRPK3, FBXO6, TARDBP, LYN, AICDA, PAN2, vif, WHSC1, VCAM1, ITGA4, IL7R, FN1, CSNK2A1, YWHAE, SMURF1, H2AFX, APTX, ESR1, FTSJ3, PCDHA2, RRP7A, R
Protein Size: 783

Poids moléculaire: 86 kDa

ID gène: 9188

NCBI Accession: NM_004728, NP_004719

UniProt: Q9NR30

Pathways: SARS-CoV-2 Protein Interactome