MGMT anticorps (Middle Region)

N° du produit ABIN2777118

Cet anticorps anti-MGMT est un anticorps Lapin Polyclonal détectant MGMT dans WB. Adapté pour Humain, Rat, Souris, Chien, Cobaye, Cheval, Lapin et Saccharomyces cerevisiae.

Aperçu rapide pour MGMT anticorps (Middle Region) (ABIN2777118)

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivité: Humain, Rat, Souris, Chien, Cobaye, Cheval, Lapin, Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MGMT est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-MGMT anticorps

Épitope: Middle Region

Séquence: ISYQQLAALA GNPKAARAVG GAMRGNPVPI LIPCHRVVCS SGAVGNYSGG

Homologie: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%

Attributs du produit: This is a rabbit polyclonal antibody against MGMT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human MGMT

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 207 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur MGMT

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Autre désignation: MGMT

Sujet: MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA.MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Alias Symbols: -
Protein Interaction Partner: UBC, BRCA2, ANXA2, ANXA1, SLC25A6, SLC25A4, ACTB, CCNB1, FBL, EEF1B2, EEF1A2, EEF1A1, DDX5, CDKN1A, CDK2, CDK1, H2BFM, FBXO27, TUBA1C, ANP32E, IL25, DDX24, CDK12, FBXO2, USP19, TUBB4B, PRPF4, RNF8, EIF3H, CDK10, HIST1H4A, HIST1H2AM, HIST3H3, SMC1A, YWHAZ,
Protein Size: 207

Poids moléculaire: 22 kDa

ID gène: 4255

NCBI Accession: NM_002412, NP_002403

UniProt: P16455

Pathways: Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus