BRCA1 anticorps (Middle Region)

N° du produit ABIN2777874

Cet anticorps Lapin Polyclonal détecte spécifiquement BRCA1 dans WB et IHC. Il présente une réactivité envers Humain, Rat, Souris et Boeuf (Vache) et a été mentionné dans 1 publication.

Aperçu rapide pour BRCA1 anticorps (Middle Region) (ABIN2777874)

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivité: Humain, Rat, Souris, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BRCA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-BRCA1 anticorps

Épitope: Middle Region

Séquence: DDLLDDGEIK EDTSFAENDI KESSAVFSKS VQKGELSRSP SPFTHTHLAQ

Homologie: Cow: 75%, Human: 100%, Mouse: 79%, Rat: 86%

Attributs du produit: This is a rabbit polyclonal antibody against BRCA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human BRCA1

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 1863 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-BRCA1 (ABIN2777874)

Evans, Shenton, Woodward, Lalloo, Howell, Maher: "Penetrance estimates for BRCA1 and BRCA2 based on genetic testing in a Clinical Cancer Genetics service setting: risks of breast/ovarian cancer quoted should reflect the cancer burden in the family." dans: BMC cancer, Vol. 8, pp. 155, (2008) (PubMed).

Détails sur BRCA1

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Autre désignation: BRCA1

Sujet: The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. BRCA1 plays a central role in DNA repair by facilitating cellular response to DNA repair. BRCA1 is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. BRCA1 is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is also required for FANCD2 targeting to sites of DNA damage.It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified.
Alias Symbols: BRCAI, BRCC1, IRIS, PSCP, RNF53, PNCA4, BROVCA1, PPP1R53
Protein Interaction Partner: RAD50, MED17, RAD51, YAP3, HDA3, SSN3, BEM4, GYP5, YAF9, HDAC2, MSH6, BAP1, SUPT5H, POLR2A, NPR1, NUP53, MET18, CDC21, MLP1, CTK1, MSH2, BACH1, DAN1, TMA22, BBC1, RPB4, SPT4, YGR053C, ATE1, YBP2, SLI15, RAD16, XRS2, RAD55, BUR2, BRIP1, ESR1, BECN1, HIST1H
Protein Size: 1863

Poids moléculaire: 208 kDa

ID gène: 672

NCBI Accession: NM_007294, NP_009225

UniProt: P38398

Pathways: Cycle Cellulaire, Réparation de l'ADN, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus