INSIG1 anticorps (Middle Region)

N° du produit ABIN2778638

Cet anticorps Lapin Polyclonal détecte spécifiquement INSIG1 dans WB. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Chévre, Cheval, Lapin, Chien, Cobaye, Mouton, Porc et Poisson zèbre (Danio rerio).

Aperçu rapide pour INSIG1 anticorps (Middle Region) (ABIN2778638)

Antigène: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chévre, Cheval, Lapin, Chien, Cobaye, Mouton, Porc, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp INSIG1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-INSIG1 anticorps

Épitope: Middle Region

Séquence: WWVPPCCGTA SAVIGLLYPC IDRHLGEPHK FKREWSSVMR CVAVFVGINH

Homologie: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 87%

Attributs du produit: This is a rabbit polyclonal antibody against INSIG1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human INSIG1

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 225 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur INSIG1

Antigène: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Autre désignation: INSIG1

Sujet: Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. INSIG1 is an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. INSIG1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins.The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MGC26273, INSIG1
Protein Interaction Partner: RNF5, HMGCR, SCAP, SREBF1, RNF139, UBQLN1, UBQLN2, FAF2, ADRM1, PSMD14, BAG6, VCP, UBC, RPN1, PSMD13, PSMD12, PSMD11, PSMD4, PSMC6, PSMC5, PSMC3, PSMC2, PSMB3, PSMB1, PSMA7, PSMA2, LGALS7, JUP, INSIG1, HSPA8, HSPA6, HSPA5, HSPA1A, ENO1, DSP, AMFR, SREBF2,
Protein Size: 225

Poids moléculaire: 25 kDa

ID gène: 51141

NCBI Accession: NM_016133, NP_057217

UniProt: Q9Y5U4

Pathways: ER-Nucleus Signaling