PTBP1 anticorps (Middle Region)

N° du produit ABIN2778815

Cet anticorps anti-PTBP1 est un anticorps Lapin Polyclonal détectant PTBP1 dans WB. Adapté pour Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin et Poisson zèbre (Danio rerio). Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour PTBP1 anticorps (Middle Region) (ABIN2778815)

Antigène: PTBP1 (Polypyrimidine Tract Binding Protein 1 (PTBP1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PTBP1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PTBP1 anticorps

Épitope: Middle Region

Séquence: KGFKFFQKDR KMALIQMGSV EEAVQALIDL HNHDLGENHH LRVSFSKSTI

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Attributs du produit: This is a rabbit polyclonal antibody against PTBP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human PTBP1

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 557 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-PTBP1 (ABIN2778815)

Clower, Chatterjee, Wang, Cantley, Vander Heiden, Krainer: "The alternative splicing repressors hnRNP A1/A2 and PTB influence pyruvate kinase isoform expression and cell metabolism." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 5, pp. 1894-9, (2010) (PubMed).

Détails sur PTBP1

Antigène: PTBP1 (Polypyrimidine Tract Binding Protein 1 (PTBP1))

Autre désignation: PTBP1

Sujet: PTBP1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Alias Symbols: HNRNP-I, HNRNPI, HNRPI, MGC10830, MGC8461, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB
Protein Interaction Partner: UBC, SNRPA, SFPQ, PTBP1, NONO, LGR4, MDM2, RPA3, RPA2, RPA1, BMI1, SUZ12, EED, RNF2, DIMT1, YBX3, AIMP2, SPTAN1, RPS27, QARS, YBX1, RPSA, ILF2, HNRNPU, HNRNPD, HNRNPA2B1, FAU, EPRS, EIF4G1, APBB1, ABCF1, NELFB, G3BP1, AIMP1, EIF2B2, IQGAP1, TARDBP, SRPK2,
Protein Size: 557

Poids moléculaire: 60 kDa

ID gène: 5725

NCBI Accession: NM_002819, NP_002810

UniProt: Q29099

Pathways: Regulation of Muscle Cell Differentiation