Sonic Hedgehog anticorps (N-Term)

N° du produit ABIN2781775

L’anticorps Lapin Polyclonal anti-Sonic Hedgehog a été validé pour WB et IHC. Il convient pour détecter Sonic Hedgehog dans des échantillons de Humain, Souris, Rat, Poisson zèbre (Danio rerio), Chien, Boeuf (Vache), Cobaye, Cheval, Chévre et Lapin.

Aperçu rapide pour Sonic Hedgehog anticorps (N-Term) (ABIN2781775)

Antigène: Sonic Hedgehog (SHH)

Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio), Chien, Boeuf (Vache), Cobaye, Cheval, Chévre, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Sonic Hedgehog est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-Sonic Hedgehog anticorps

Épitope: N-Term

Séquence: RCLLLVLVSS LLVCSGLACG PGRGFGKRRH PKKLTPLAYK QFIPNVAEKT

Homologie: Cow: 93%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Attributs du produit: This is a rabbit polyclonal antibody against SHH. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human SHH

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 462 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur Sonic Hedgehog

Antigène: Sonic Hedgehog (SHH)

Autre désignation: SHH

Sujet: SHH is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in its gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.This gene, which is expressed only during embryogenesis, encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.
Alias Symbols: HHG1, HLP3, HPE3, SMMCI, TPT, TPTPS, MCOPCB5
Protein Interaction Partner: UBC, SEL1L, DERL2, DERL1, SYVN1, HHIP, PTCH2, PTCH1, SHH, ADCYAP1, GAS1,
Protein Size: 462

Poids moléculaire: 28 kDa

ID gène: 6469

NCBI Accession: NM_000193, NP_000184

UniProt: Q15465

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development