FEN1 anticorps (C-Term)

N° du produit ABIN2782483

Cet anticorps Lapin Polyclonal détecte spécifiquement FEN1 dans WB. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Mouton, Cheval, Chien, Cobaye, Lapin et Poisson zèbre (Danio rerio).

Aperçu rapide pour FEN1 anticorps (C-Term) (ABIN2782483)

Antigène: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Mouton, Cheval, Chien, Cobaye, Lapin, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FEN1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-FEN1 anticorps

Épitope: C-Term

Séquence: PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 93%

Attributs du produit: This is a rabbit polyclonal antibody against FEN1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogène: The immunogen is a synthetic peptide directed towards the C terminal region of human FEN1

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 380 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur FEN1

Antigène: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Autre désignation: FEN1

Sujet: FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Alias Symbols: FEN-1, MF1, RAD2
Protein Interaction Partner: SUMO2, SUMO3, PCNA, UBC, SHFM1, ELAC2, TNPO3, PPP2R5D, HARS, LPP, PFDN1, WRN, AICDA, RAD1, FN1, PAXIP1, APP, VRK1, RAP1A, cdt1, TERF1, RAD21, GSK3B, ENDOG, DDX11, NCAPG, EP300, BLM, EEF1G, ARHGDIA, HNRNPA1, CDK2, CDK1, APEX1, RAD9A, HUS1, CCNA2,
Protein Size: 380

Poids moléculaire: 42 kDa

ID gène: 2237

NCBI Accession: NM_004111, NP_004102

UniProt: P39748

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA