POLR3B anticorps (C-Term)

N° du produit ABIN2783516

Il existe 1 publication pour ce produit. L’anticorps anti-POLR3B Polyclonal Lapin est utilisé pour la détection de POLR3B dans des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache), Lapin, Poisson zèbre (Danio rerio), Cheval, Cobaye, Saccharomyces cerevisiae et Chévre. Il a été validé pour WB.

Aperçu rapide pour POLR3B anticorps (C-Term) (ABIN2783516)

Antigène: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Lapin, Poisson zèbre (Danio rerio), Cheval, Cobaye, Saccharomyces cerevisiae, Chévre

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR3B est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-POLR3B anticorps

Épitope: C-Term

Fonction: POLR3B Antibody - C-terminal region

Séquence: IEKVMISSNA EDAFLIKMLL RQTRRPEIGD KFSSRHGQKG VCGLIVPQED

Homologie: Cow: 100%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%

Attributs du produit: This is a rabbit polyclonal antibody against POLR3B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the C terminal region of human POLR3B

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48821-100UG

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-POLR3B (ABIN2783516)

Yee, Gong, Huang, Lorent, Dolan, Maraia, Pack: "Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and disrupts zebrafish digestive development." dans: PLoS biology, Vol. 5, Issue 11, pp. e312, (2007) (PubMed).

Détails sur POLR3B

Antigène: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Autre désignation: POLR3B

Sujet:

Targeted Subunit: RPC2

Background Information: POLR3B belongs to the RNA polymerase beta chain family. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3B is the second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It is proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.

Gene Name: Polymerase (RNA) III (DNA directed) polypeptide B

Alternative Symbols: C128, HLD8, RPC2, INMAP

Protein Name: DNA-directed RNA polymerase III subunit RPC2

Poids moléculaire: 128kDa

ID gène: 55703

NCBI Accession: NP_060552

UniProt: Q9NW08