ADD2 anticorps (Middle Region)

N° du produit ABIN2784900

Cet anticorps Lapin Polyclonal détecte spécifiquement ADD2 dans WB et IF. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Cheval, Porc, Chien, Cobaye et Lapin.

Aperçu rapide pour ADD2 anticorps (Middle Region) (ABIN2784900)

Antigène: ADD2 (Adducin 2 (Beta) (ADD2))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cheval, Porc, Chien, Cobaye, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ADD2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-ADD2 anticorps

Épitope: Middle Region

Séquence: VVNGREEEQT AEEILSKGLS QMTTSADTDV DTSKDKTESV TSGPMSPEGS

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 79%, Pig: 100%, Rabbit: 93%, Rat: 100%

Attributs du produit: This is a rabbit polyclonal antibody against ADD2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human ADD2

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 726 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur ADD2

Antigène: ADD2 (Adducin 2 (Beta) (ADD2))

Autre désignation: ADD2

Sujet: Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known.
Alias Symbols: ADDB
Protein Interaction Partner: UBC, APP, RPH3A, PRKACA, PRKCD, PTPRZ1, FYN, CALM1, ADD2, ADD1,
Protein Size: 726

Poids moléculaire: 80 kDa

ID gène: 119

NCBI Accession: NM_001617, NP_001608

UniProt: P35612

Pathways: Regulation of Actin Filament Polymerization