NAE1 anticorps (C-Term)

N° du produit ABIN2788045

Cet anticorps Lapin Polyclonal détecte spécifiquement NAE1 dans WB. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Porc, Lapin et Poisson zèbre (Danio rerio).

Aperçu rapide pour NAE1 anticorps (C-Term) (ABIN2788045)

Antigène: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Porc, Lapin, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NAE1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-NAE1 anticorps

Épitope: C-Term

Fonction: Nae1 Antibody - C-terminal region

Séquence: ARALKEFVAK EGQGNLPVRG TIPDMIADSN KYIKLQNVYR EKAKKDAAAV

Homologie: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Attributs du produit: This is a rabbit polyclonal antibody against Nae1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the C-terminal region of Mouse Nae1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP59379-100UG

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Détails sur NAE1

Antigène: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Autre désignation: Nae1

Sujet:

Background Information: Nae1 is a regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

Gene Name: NEDD8 activating enzyme E1 subunit 1

Alternative Symbols: Ap, 59 kDa, Appbp1

Protein Name: NEDD8-activating enzyme E1 regulatory subunit

Poids moléculaire: 60kDa

ID gène: 234664

NCBI Accession: NP_659180

UniProt: Q8VBW6