PCNA anticorps (C-Term)

N° du produit ABIN2792157

Cet anticorps Lapin Polyclonal détecte spécifiquement PCNA dans WB et IHC. Il présente une réactivité envers Humain, Souris, Rat, Chien, Poisson zèbre (Danio rerio) et Boeuf (Vache) et a été mentionné dans 1 publication.

Aperçu rapide pour PCNA anticorps (C-Term) (ABIN2792157)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Souris, Rat, Chien, Poisson zèbre (Danio rerio), Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-PCNA anticorps

Épitope: C-Term

Séquence: LNFFTKATPL SSTVTLSMSA DVPLVVEYKI ADMGHLKYYL APKIEDEEGS

Homologie: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 91%

Attributs du produit: This is a rabbit polyclonal antibody against PCNA. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogène: The immunogen is a synthetic peptide directed towards the C terminal region of human PCNA

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 261 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-PCNA (ABIN2792157)

Liu, Meng, Li, Zhou, Zeng, He, Mrsny, Liu, Hu, Hu, Li: "Human umbilical cord mesenchymal stromal cells rescue mice from acetaminophen-induced acute liver failure." dans: Cytotherapy, Vol. 16, Issue 9, pp. 1207-19, (2014) (PubMed).

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet: PCNA is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Alias Symbols: MGC8367
Protein Interaction Partner: GADD45A, CDKN1A, HUWE1, POLH, PCNA, MSH3, FUS, UBC, TMEM218, CHFR, DTL, POLE, POLD1, MCM7, MCM4, LIG1, MSH6, FEN1, FBXO18, ZRANB3, CHTF18, DNMT1, RAD18, SIVA1, KIAA0101, TDG, SUMO2, SUMO3, RPA1, RFC5, RFC4, RFC3, RFC2, RFC1, MDM2, TRIM25, USP18, ISG15, MA
Protein Size: 261

Poids moléculaire: 29 kDa

ID gène: 5111

NCBI Accession: NM_002592, NP_002583

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy