E2F1 anticorps (N-Term)

N° du produit ABIN2792603

Détail du produit anti-E2F1 anticorps

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Chien, Cobaye, Lapin, Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp E2F1 est non-conjugé

Application: Western Blotting (WB)

Séquence: PARGRGRHPG KGVKSPGEKS RYETSLNLTT KRFLELLSHS ADGVVDLNWA

Réactivité croisée: Boeuf (Vache), Chien, Poulet, Humain, Souris, Rat (Rattus)

Homologie: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Attributs du produit: This is a rabbit polyclonal antibody against E2F1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human E2F1

Information d'application

Indications d'application: WB Suggested Anti-E2F1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:1562500
Positive Control: Transfected 293T.
Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 437 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur E2F1

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Autre désignation: E2F1 (E2F1 Produits)

Synonymes: anticorps CG6376, anticorps DRTF1/E2F, anticorps DmE2F-1, anticorps Dmel\\CG6376, anticorps Dp, anticorps E(Sev-CycE)3A, anticorps E(var)3-93E, anticorps E(var)3-95E, anticorps E(var)93E, anticorps E2F, anticorps E2F-1, anticorps E2F1, anticorps E2f1, anticorps Evar(3)164, anticorps dE2F, anticorps dE2F1, anticorps dE2f, anticorps dE2f1, anticorps de2f1, anticorps def21, anticorps drosE2F1, anticorps e2f, anticorps e2f1, anticorps l(3)07172, anticorps l(3)j3B1, anticorps l(3)j3C2, anticorps l(3)rM729, anticorps RBAP1, anticorps RBBP3, anticorps RBP3, anticorps mKIAA4009, anticorps xE2F, anticorps ATE2FB, anticorps E2F TRANSCRIPTION FACTOR-1 E2F1, anticorps E2F transcription factor 1, anticorps E2FB, anticorps E2FB TRANSCRIPTION FACTOR, anticorps E2F transcription factor 1, anticorps E2F transcription factor 1 L homeolog, anticorps E2f1, anticorps E2F1, anticorps e2f1.L

Sujet: The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: E2F-1, RBBP3, RBP3, RBAP1
Protein Interaction Partner: RB1, SIRT1, KDM2A, LAMTOR5, EP300, KAT2B, VHL, RARA, STAT1, NCOR2, STOML1, RBL1, MAGEA11, SKP2, UHRF2, UBC, GTF2H1, ERCC3, SPT15, TFB1, TBP, NCOA3, CEBPE, PARP1, PRMT2, RBL2, CCNE1, TFDP2, TFDP1, CDKN2A, CEBPD, CDK2, CDK1, NEDD8, MCPH1, DDB2, DDB1, XIAP,
Protein Size: 437

Poids moléculaire: 47 kDa

ID gène: 1869

NCBI Accession: NM_005225, NP_005216

UniProt: Q01094

Pathways: Signalisation p53, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy