CD33 anticorps (N-Term)

N° du produit ABIN2798973

Cet anticorps anti-CD33 est un anticorps Lapin Polyclonal détectant CD33 dans WB et IHC. Adapté pour Humain.

Aperçu rapide pour CD33 anticorps (N-Term) (ABIN2798973)

Antigène: CD33

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD33 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-CD33 anticorps

Épitope: AA 12-39, N-Term

Fonction: Rabbit Anti-Human CD33 (SIGLEC3) (N-term) Antibody

Immunogène: This CD33 (SIGLEC3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human CD33 (SIGLEC3).

Isotype: Ig Fraction

Information d'application

Indications d'application: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50The anti-Siglec3 N-term Pab , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2.0 mg/mL

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C (short-term), -20°C (long-term)

Détails sur CD33

Antigène: CD33

Autre désignation: CD33

Sujet:

Target Description: SIGLEC3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,6-linked sialic acid, the sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, SIGLEC3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. This protein Induces apoptosis in acute myeloid leukemia (in vitro). It has been shown to interact with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. SIGLEC3 expresses in monocytic/myeloid lineage cells, and contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. The gene for SIGLEC3 belongs to the immunoglobulin superfamily.

Gene Symbol: CD33

Poids moléculaire: 39825 Da

ID gène: 945

UniProt: P20138