CDKN1B anticorps

N° du produit ABIN2854753

Cet anticorps Lapin Polyclonal détecte spécifiquement CDKN1B dans WB, IHC, IF, IHC (p), IP, ICC et IHC (fro). Il présente une réactivité envers Humain et a été mentionné dans 3+ publications.

Aperçu rapide pour CDKN1B anticorps (ABIN2854753)

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDKN1B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Classe de qualité: KO Validated

Détail du produit anti-CDKN1B anticorps

Specificité: IP/MS validation was supported by references (PMID:30377371)

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Rabbit Polyclonal antibody to p27 Kip1 (cyclin-dependent kinase inhibitor 1B (p27, Kip1))
p27 Kip1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Validation: IP/MS, KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.08 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-CDKN1B (ABIN2854753)

Scully, Lahmy, Signaevskaia, Sasik, Medal, Kim, French, James, Wu, Lowy, Itkin-Ansari: "E47 Governs the MYC-CDKN1B/p27KIP1-RB Network to Growth Arrest PDA Cells Independent of CDKN2A/p16INK4A and Wild-Type p53." dans: Cellular and molecular gastroenterology and hepatology, Vol. 6, Issue 2, pp. 181-198, (2018) (PubMed).

Mlcochova, Caswell, Taylor, Towers, Gupta: "DNA damage induced by topoisomerase inhibitors activates SAMHD1 and blocks HIV-1 infection of macrophages." dans: The EMBO journal, Vol. 37, Issue 1, pp. 50-62, (2018) (PubMed).

Xu, Wu, Zheng, Yu, Yang, Yao, Zhou, Ching, Lau: "Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells." dans: Oncotarget, Vol. 7, Issue 5, pp. 6146-58, (2017) (PubMed).

Détails sur CDKN1B

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Autre désignation: cyclin dependent kinase inhibitor 1B

Sujet: This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Cellular Localization: Nucleus , Cytoplasm

Poids moléculaire: 22 kDa

ID gène: 1027

UniProt: P46527

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy