BRCA1 anticorps (C-Term)

N° du produit ABIN2854845

Cet anticorps anti-BRCA1 est un anticorps Lapin Polyclonal détectant BRCA1 dans ELISA, IF et ICC. Adapté pour Humain.

Aperçu rapide pour BRCA1 anticorps (C-Term) (ABIN2854845)

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BRCA1 est non-conjugé

Application: ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-BRCA1 anticorps

Épitope: C-Term

Réactivité croisée: Chien, Macaque rhésus, Chimpanzé

Réactivité croisée (Details): Dog (87 %), Rhesus Monkey (100 %), Chimpanzee (100 %)

Attributs du produit: Rabbit Polyclonal antibody to BRCA1 (breast cancer 1, early onset)
BRCA1 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogène: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BRCA1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference ICC/IF 1:100-1:1000* ELISA-Ag Assay-dependent dilution Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* ELISA-AgAssay-dependent dilution

Commentaires:

Positive Control: Synthetic peptide: 1:8,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur BRCA1

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Autre désignation: BRCA1

Sujet: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified.

Cellular Localization: Nucleus , Isoform 3: Cytoplasm , Isoform 5: Cytoplasm

Poids moléculaire: 208 kDa

ID gène: 672

Pathways: Cycle Cellulaire, Réparation de l'ADN, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus