14-3-3 sigma/SFN anticorps

N° du produit ABIN2854867

Cet anticorps Lapin Polyclonal détecte spécifiquement 14-3-3 sigma/SFN dans WB, IF, IP, IHC (p) et ICC. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour 14-3-3 sigma/SFN anticorps (ABIN2854867)

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp 14-3-3 sigma/SFN est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-14-3-3 sigma/SFN anticorps

Réactivité croisée: Humain, Souris

Attributs du produit: Rabbit Polyclonal antibody to 14-3-3 sigma (stratifin)
14-3-3 sigma antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human 14-3-3 sigma. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: A549 , 293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8D30

Validation: Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.32 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-14-3-3 sigma/SFN (ABIN2854867)

Kim, Morales, Baek, Slaga, DiGiovanni, Kim: "UVB-induced nuclear translocation of TC-PTP by AKT/14-3-3σ axis inhibits keratinocyte survival and proliferation." dans: Oncotarget, Vol. 8, Issue 53, pp. 90674-90692, (2017) (PubMed).

Détails sur 14-3-3 sigma/SFN

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Autre désignation: stratifin

Sujet: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. p53-regulated inhibitor of G2/M progression.

Cellular Localization: Cytoplasm , Nucleus , Secreted

Poids moléculaire: 28 kDa

ID gène: 2810

UniProt: P31947

Pathways: Signalisation p53, Myometrial Relaxation and Contraction