FEN1 anticorps

N° du produit ABIN2855166

Cet anticorps anti-FEN1 est un anticorps Lapin Polyclonal détectant FEN1 dans WB, IHC, IF, IP, IHC (p), ICC et PLA. Adapté pour Humain.

Aperçu rapide pour FEN1 anticorps (ABIN2855166)

Antigène: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FEN1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Proximity Ligation Assay (PLA)

Détail du produit anti-FEN1 anticorps

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Rabbit Polyclonal antibody to FEN1 (flap structure-specific endonuclease 1)
FEN1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human FEN1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: NIH-3T3 , JC , BCL-1 , Raw264.7 , PC-12

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur FEN1

Antigène: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Autre désignation: flap structure-specific endonuclease 1

Sujet: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Cellular Localization: Nucleus

Poids moléculaire: 43 kDa

ID gène: 2237

UniProt: P39748

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA