XBP1 anticorps

N° du produit ABIN2855334

Cet anticorps anti-XBP1 est un anticorps Lapin Polyclonal détectant XBP1 dans WB, IF, ICC et IHC (p). Adapté pour Humain.

Aperçu rapide pour XBP1 anticorps (ABIN2855334)

Antigène: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp XBP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-XBP1 anticorps

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Rabbit polyclonal antibody to XBP1 (X-box binding protein 1), which recognizes XBP-1U (unspliced), or isoform 1.
XBP1 antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human XBP1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Validation: Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.93 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur XBP1

Antigène: XBP1 (X-Box Binding Protein 1 (XBP1))

Autre désignation: X-box binding protein 1

Sujet: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

Cellular Localization: Nucleus

Poids moléculaire: 29 kDa

ID gène: 7494

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response