MDC1 anticorps

N° du produit ABIN2855454

Cet anticorps anti-MDC1 est un anticorps Lapin Polyclonal détectant MDC1 dans WB, IF, IHC (p) et ICC. Adapté pour Humain. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour MDC1 anticorps (ABIN2855454)

Antigène: MDC1 (Mdc1 (MDC1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MDC1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-MDC1 anticorps

Réactivité croisée: Humain

Attributs du produit: Rabbit Polyclonal antibody to MDC1 (mediator of DNA-damage checkpoint 1)
MDC1 antibody [N2N3]

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human MDC1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: HeLa nucleus

Validation: Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-MDC1 (ABIN2855454)

Burger, Schlackow, Gullerova: "Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks." dans: Nucleic acids research, Vol. 47, Issue 7, pp. 3467-3484, (2019) (PubMed).

Chen, Tsai, Wang, Teng: "SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression." dans: Scientific reports, Vol. 7, Issue 1, pp. 3842, (2017) (PubMed).

Détails sur MDC1

Antigène: MDC1 (Mdc1 (MDC1))

Autre désignation: mediator of DNA damage checkpoint 1

Sujet: The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

Cellular Localization: Nucleus

Poids moléculaire: 227 kDa

ID gène: 9656

UniProt: Q14676