Cyclin A anticorps

N° du produit ABIN2855543

L’anticorps Lapin Polyclonal anti-Cyclin A a été validé pour WB, IF, IP, IHC (p) et ICC. Il convient pour détecter Cyclin A dans des échantillons de Humain. Il y a 5+ publications disponibles.

Aperçu rapide pour Cyclin A anticorps (ABIN2855543)

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Classe de qualité: KO Validated

Détail du produit anti-Cyclin A anticorps

Specificité: IP/MS validation was supported by references (PMID:30377384)

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Rabbit Polyclonal antibody to Cyclin A2 (cyclin A2)
Cyclin A2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: Neuro2A , PC-12 , IMR32 , SK-N-AS , HeLa(100 μg/ml Bleomycin treatment for 24 hr)

Validation: IP/MS, KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.73 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-Cyclin A (ABIN2855543)

Honda, Inui: "PDZRN3 protects against apoptosis in myoblasts by maintaining cyclin A2 expression." dans: Scientific reports, Vol. 10, Issue 1, pp. 1140, (2020) (PubMed).

Ogawa, Yaginuma, Nakatomi, Nakajima, Tada-Shigeyama, Addison, Urata, Matsubara, Watanabe, Matsuo, Sato, Honda, Hikiji, Watanabe, Kokabu: "Transducin-like enhancer of split 3 regulates proliferation of melanoma cells via histone deacetylase activity." dans: Oncotarget, Vol. 10, Issue 3, pp. 404-414, (2019) (PubMed).

Fu, Xu, Chen, Li, Zhao, Chen: "Microarray analysis reveals Tmub1 as a cell cycle-associated protein in rat hepatocytes." dans: Molecular medicine reports, Vol. 17, Issue 3, pp. 4337-4344, (2018) (PubMed).

Li, Wu, Lee, Li, Hsieh, Ho, Ho, Chen, Wang, Yen, Yang, Chen: "Hinokitiol induces DNA damage and autophagy followed by cell cycle arrest and senescence in gefitinib-resistant lung adenocarcinoma cells." dans: PLoS ONE, Vol. 9, Issue 8, pp. e104203, (2015) (PubMed).

Lin, Chou, Lee, Chen, Fong, Chang, Huang, Wu, Chang, Wang, Chiu: "The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB." dans: Cancer cell international, Vol. 14, Issue 1, pp. 1, (2014) (PubMed).

Détails sur Cyclin A

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Autre désignation: cyclin A2

Sujet: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Cellular Localization: Nucleus , Cytoplasm

Poids moléculaire: 49 kDa

ID gène: 890

UniProt: P20248

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA