GRP94 anticorps (Center)

N° du produit ABIN2855578

Cet anticorps Lapin Polyclonal détecte spécifiquement GRP94 dans WB, IF, IP, ICC et IHC (p). Il présente une réactivité envers Humain et Souris et a été mentionné dans 1 publication.

Aperçu rapide pour GRP94 anticorps (Center) (ABIN2855578)

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GRP94 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GRP94 anticorps

Épitope: Center

Réactivité croisée: Macaque rhésus, Chimpanzé

Réactivité croisée (Details): Rhesus Monkey (100 %), Chimpanzee (100 %)

Attributs du produit: Rabbit Polyclonal antibody to GRP94 (heat shock protein 90 kDa beta (Grp94), member 1)
GRP94 antibody [N1N3]

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human GRP94. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

Commentaires:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 , *endothelial cells

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.95 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-GRP94 (ABIN2855578)

Plate, Cooley, Chen, Paxman, Gallagher, Madoux, Genereux, Dobbs, Garza, Spicer, Scampavia, Brown, Rosen, Powers, Walter, Hodder, Wiseman, Kelly: "Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation." dans: eLife, Vol. 5, (2017) (PubMed).

Détails sur GRP94

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Autre désignation: GRP94

Sujet: HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1, MIM 140571) and mitochondria (TRAP1, MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Cellular Localization: Endoplasmic reticulum lumen , Melanosome

Poids moléculaire: 92 kDa

ID gène: 7184

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades