PRKAA2 anticorps (Center)
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- Antigène Voir toutes PRKAA2 Anticorps
- PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))
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Épitope
- Center
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Reactivité
- Humain, Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PRKAA2 est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Réactivité croisée
- Poulet, Porc, Rat (Rattus), Boeuf (Vache), Xenopus tropicalis
- Réactivité croisée (Details)
- Chicken (96 %), Pig (99 %), Rat (98 %), Bovine (99 %), Xenopus tropicalis (96 %)
- Attributs du produit
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Rabbit Polyclonal antibody to AMPK alpha 2 (protein kinase, AMP-activated, alpha 2 catalytic subunit)
AMPK alpha 2 antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogène
- Recombinant protein encompassing a sequence within the center region of human AMPK alpha 2. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product PRKAA2 Anticorps primaire
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- Indications d'application
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
- Commentaires
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Positive Control: A431 , HeLa , A375 , NIH-3T3
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.55 mg/mL
- Buffer
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Agent conservateur
- Thimerosal (Merthiolate)
- Précaution d'utilisation
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2." dans: Redox biology, Vol. 28, pp. 101345, (2020) (PubMed).
: "Alternative polyadenylation drives genome-to-phenome information detours in the AMPKα1 and AMPKα2 knockout mice." dans: Scientific reports, Vol. 8, Issue 1, pp. 6462, (2018) (PubMed).
: "Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK." dans: Nature, Vol. 482, Issue 7384, pp. 251-5, (2012) (PubMed).
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Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2." dans: Redox biology, Vol. 28, pp. 101345, (2020) (PubMed).
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- Antigène
- PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))
- Autre désignation
- AMPK alpha 2 (PRKAA2 Produits)
- Synonymes
- anticorps AMPK, anticorps AMPK2, anticorps AMPKa2, anticorps PRKAA, anticorps Ampk, anticorps Ampka2, anticorps 2310008I11Rik, anticorps A830082D05, anticorps AMPKalpha2, anticorps AMPKA2, anticorps ampk, anticorps ampk2, anticorps prkaa, anticorps prkaa1, anticorps PRKAA2, anticorps protein kinase AMP-activated catalytic subunit alpha 2, anticorps protein kinase, AMP-activated, alpha 2 catalytic subunit, anticorps protein kinase, AMP-activated, alpha 2 catalytic subunit S homeolog, anticorps PRKAA2, anticorps Prkaa2, anticorps prkaa2.S, anticorps prkaa2
- Sujet
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The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
- Poids moléculaire
- 62 kDa
- ID gène
- 5563
- Pathways
- AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, L'effet Warburg
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