VPS35 anticorps (C-Term)

N° du produit ABIN2856562

Cet anticorps Lapin Polyclonal détecte spécifiquement VPS35 dans WB, IF, ICC et IHC (p). Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 1 publication.

Aperçu rapide pour VPS35 anticorps (C-Term) (ABIN2856562)

Antigène: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VPS35 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-VPS35 anticorps

Épitope: C-Term

Réactivité croisée: Poisson zèbre (Danio rerio), Poulet, Boeuf (Vache)

Réactivité croisée (Details): Zebrafish (86 %), Chicken (93 %), Bovine (100 %)

Attributs du produit: Rabbit Polyclonal antibody to VPS35 (vacuolar protein sorting 35 homolog (S. cerevisiae))
VPS35 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogène: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Commentaires:

Positive Control: A549 , H1299 , HCT116 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain , rat brain

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-VPS35 (ABIN2856562)

Lenoir, Ustunel, Rajesh, Kaur, Moreau, Gruenberg, Overduin: "Phosphorylation of conserved phosphoinositide binding pocket regulates sorting nexin membrane targeting." dans: Nature communications, Vol. 9, Issue 1, pp. 993, (2018) (PubMed).

Détails sur VPS35

Antigène: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Autre désignation: VPS35

Sujet: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Cellular Localization: Cytoplasm , Membrane, Peripheral membrane protein

Poids moléculaire: 92 kDa

ID gène: 55737