Aurora A anticorps (AA 394-403)

N° du produit ABIN292641

Cet anticorps anti-Aurora A Polyclonal Chèvre (ABIN292641) détecte spécifiquement Aurora A dans WB, ELISA, IHC, IHC (p) et IP. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Aurora A anticorps (AA 394-403) (ABIN292641)

Antigène: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Aurora A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Détail du produit anti-Aurora A anticorps

Épitope: AA 394-403

Specificité: Human AURKA / Aurora-A. Reported variants represent identical protein: NP_003591.2, NP_940835.1, NP_940836.1, NP_940837.1, NP_940838.1, NP_940839.1

Homologie: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon, Marmoset (90%) Sheep, Bovine, Elephant, Panda, Helicobacter (80%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-QNKESASKQS from the C-terminus of human AURKA / Aurora-A (NP_003591.2). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Marmoset (90%), Sheep, Bovine, Elephant, Panda, Helicobacter (80%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), IP, Peptide-ELISA, WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Aurora A

Antigène: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Autre désignation: AURKA / Aurora-A

Sujet: Name/Gene ID: AURKA
Subfamily: Aurora/IPL1-like
Family: Protein Kinase

Synonyms: AURKA, AIK, AIRK1, ARK1, Aurora-A kinase, Aurora kinase A, Aurora/IPL1-like kinase, AYK1, AURA, AURORA2, Breast tumor-amplified kinase, Breast-tumor-amplified kinase, BTAK, HARK1, IPL1-related kinase, Serine/threonine kinase 6, STK7, STK6, PPP1R47, ARK-1, Aurora 2, Aurora-A, Aurora-related kinase 1, Aurora/IPL1-related kinase 1, IAK1, Serine/threonine kinase 15, STK15

ID gène: 6790

NCBI Accession: NP_003591

UniProt: O14965

Pathways: Cycle Cellulaire, Asymmetric Protein Localization