Kallikrein 13 anticorps (AA 19-262)

N° du produit ABIN292875

Cet anticorps anti-Kallikrein 13 est un anticorps Chèvre Polyclonal détectant Kallikrein 13 dans WB, IP, ELISA et Neut. Adapté pour Humain.

Aperçu rapide pour Kallikrein 13 anticorps (AA 19-262) (ABIN292875)

Antigène: Kallikrein 13 (KLK13) (Kallikrein-Related Peptidase 13 (KLK13))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Kallikrein 13 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Neutralization (Neut)

Détail du produit anti-Kallikrein 13 anticorps

Épitope: AA 19-262

Specificité: Selected for its ability to recognize human KLK13 in direct ELISAs and Western Blots. In these formats, this antibody shows ~10 % cross-reactivity with rhKallikrein 8, and less than 5 % cross-reactivity with rhKallikrein 11, rhKallikrein 14, and rhKallikrein 15.

Purification: Affinity purified

Immunogène: NS0-derived rhKLK13 (aa19-262).

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, IP, Neut, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhKLK13 from conditioned media of transfected NS0 cells. The recovered rhKLK13 can be detected by western blot analysis using a monoclonal antibody. Neutralization: This antibody was used to inhibit/neutralize the enzymatic activity of rhKLK13. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate, Boc-VPR-AMC. 50 % of the proteolytic activity was inhibited by the antibody at approximately 8.6 μg/mL (IC50) under conditions in which the enzyme was present at 1 μg/mL and the substrate concentration was 100 M. Considering the molecular masses of the enzyme (28 kD) and the antibody (150 kD), IC50 was achieved at approximately 2:1 Molar ratio of the antibody to the enzyme. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 1.0 ng/well.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Détails sur Kallikrein 13

Antigène: Kallikrein 13 (KLK13) (Kallikrein-Related Peptidase 13 (KLK13))

Autre désignation: KLK13 / Kallikrein 13

Sujet: Name/Gene ID: KLK13
Subfamily: Serine S1
Family: Protease

Synonyms: KLK13, Kallikrein 13, Kallikrein-13, KLK-L4, KLKL4, Kallikrein-like protein 4, Kallikrein-like gene 4

ID gène: 26085

UniProt: Q9UKR3

Pathways: Système du Complément