SMAD2 anticorps (AA 234-249)

N° du produit ABIN293877

Cet anticorps anti-SMAD2 Polyclonal Lapin (ABIN293877) détecte spécifiquement SMAD2 dans WB, IHC et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Boeuf (Vache), Lapin, Poulet, Singe, Chien, Cheval, Roussette (Chauve-souris), Hamster et Xenopus laevis.

Aperçu rapide pour SMAD2 anticorps (AA 234-249) (ABIN293877)

Antigène: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Lapin, Poulet, Singe, Chien, Cheval, Roussette (Chauve-souris), Hamster, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SMAD2 anticorps

Épitope: AA 234-249

Specificité: The SMAD2 amino acid sequence used as immunogen is 100 % homologous in human (isoform 1:467 amino acids, isoform 2:437 amino acids), mouse, rat, horse, cow, chicken and Xenopus.

Homologie: Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Rabbit, Horse, Opossum, Chicken, Platypus, Lizard, Xenopus (100%) Panda, Stickleback, Pufferfish, Zebrafish (94%).

Purification: Protein G purified

Immunogène: Amino acids 234-249 (DQQLNQSMDTGSPAEL) of human SMAD2 were used as immunogen. Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Rabbit, Horse, Opossum, Chicken, Platypus, Lizard, Xenopus (100%), Panda, Stickleback, Pufferfish, Zebrafish (94%).

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur SMAD2

Antigène: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Autre désignation: SMAD2

Sujet: Name/Gene ID: SMAD2

Synonyms: SMAD2, HMAD-2, HSMAD2, JV18-1, Mad protein homolog smad2, MADR2, MADH2, SMAD 2, MAD homolog 2, Mad-related protein 2, JV18, Mother against DPP homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD family member 2

ID gène: 4087

UniProt: Q15796

Pathways: Cycle Cellulaire, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus