DLL1 anticorps (Extracellular Domain)

N° du produit ABIN295726

Cet anticorps Mouton Polyclonal détecte spécifiquement DLL1 dans WB, ELISA et IHC. Il présente une réactivité envers Rat.

Aperçu rapide pour DLL1 anticorps (Extracellular Domain) (ABIN295726)

Antigène: DLL1 (delta-Like 1 (DLL1))

Reactivité: Rat

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp DLL1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-DLL1 anticorps

Épitope: Extracellular Domain

Specificité: Recognize rat DLL1. Species sequence homology: mouse (97 % ), human (91 %), over aa18-537.

Purification: Affinity purified

Stérilité: 0.2 μm filtered

Immunogène: NS0-derived rrDLL1 extracellular domain.

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Western Blot: 0.1-0.2 μg/mL Detection limit for recombinant rat DLL1 is 5 ng/lane under non-reducing and 25 ng/lane under reducing conditions. Immunohistochemistry: 10 μg/mL Detects DLL1 in cryosections of E13.5 mouse embryos. Tissue was fixed overnight in 4 % PFA+14 %picric acid in PBS at 4? C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 min at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by fluorochrome-coupled anti-sheep lgG at RT for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL Detection limit: 0.3 ng/well.~10 % cross-reactivity with rhDLL-1 and >1 % cross reactivity with rrNotch-1.

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 40-50 % glycerol, PBS.

Concentration: Lot specific

Buffer: Lyophilized from a 0.2 um filtered solution in PBS, 5 % trehalose.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur DLL1

Antigène: DLL1 (delta-Like 1 (DLL1))

Autre désignation: DLL1

Sujet: Name/Gene ID: DLL1

Synonyms: DLL1, Delta-like 1 (Drosophila), Delta (Drosophila)-like 1, Drosophila Delta homolog 1, Delta-like protein 1, Delta1, H-Delta-1, DL1, Npa

ID gène: 28514

UniProt: O00548

Pathways: Signalisation Notch, Stem Cell Maintenance