MAPK7 anticorps (pThr218, Tyr220)

N° du produit ABIN296169

Cet anticorps anti-MAPK7 est un anticorps Mouton Polyclonal détectant MAPK7 dans WB. Adapté pour Humain.

Aperçu rapide pour MAPK7 anticorps (pThr218, Tyr220) (ABIN296169)

Antigène: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Reactivité: Humain

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp MAPK7 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-MAPK7 anticorps

Épitope: AA 213-224, pThr218, Tyr220

Specificité: Recognizes human phospho-BMK1/Erk5 (Thr218, Tyr220), Mr ~110kD. Predicted to cross-react with mouse and rat based on sequence homology.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5 (HQYFM[pT]E[pY]VATR), KLH-conjugated. The immunizing sequence is identical in mouse, 9/12aa identical in rat.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: WB

Usage: Suitable for use in Western Blot. Western Blot: 1-:250-1:500 detects phospho-BMK1/Erk5 (Thr218, Tyr220) in RIPA lysates from HeLa cells treated with sorbitol. Lysates from untreated or sorbitol-treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with either anti-phospho-BMK1/Erk5 (Thr218, Tyr220) (1:500) or anti-BMK1/Erk5 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (BMK1/Erk5 or phospho- BMK1/Erk5 (110kD).

Not recommended for: IHC-P

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur MAPK7

Antigène: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Autre désignation: MAPK7 / ERK5

Sujet: Name/Gene ID: MAPK7
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK7, Big MAP kinase 1, BMK-1, Erk4, ERK5, MAPK 7, PRKM7, BMK1, BMK1 kinase, ERK-5, MAP kinase 7

ID gène: 5598

UniProt: Q13164

Pathways: Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, cAMP Metabolic Process, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling