LAT anticorps (Cytoplasmic Domain)

N° du produit ABIN302069

Il existe 2+ publications pour ce produit. L’anticorps anti-LAT Monoclonal Souris est utilisé pour la détection de LAT dans des échantillons de Humain. Il a été validé pour WB, IP et ICFC.

Aperçu rapide pour LAT anticorps (Cytoplasmic Domain) (ABIN302069)

Antigène: LAT (Linker For Activation of T Cells (LAT))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LAT est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC)

Clone: LAT-01

Détail du produit anti-LAT anticorps

Épitope: Cytoplasmic Domain

Fonction: Anti-LAT Purified

Aucune reactivité croisée: Souris

Réactivité croisée (Details): Human

Attributs du produit: The antibody LAT-01 reacts with an intracellular epitope of LAT, a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT.

Isotype: IgG1

Information d'application

Indications d'application: Immunoprecipitation: Tyrosine-phosphorylated form of LAT is not optimally recognized.
Flow cytometry: Recommended dilution: 1-4 μg/mL, intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-LAT (ABIN302069)

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." dans: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." dans: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).

Détails sur LAT

Antigène: LAT (Linker For Activation of T Cells (LAT))

Autre désignation: LAT

Sujet: Linker for activation of T cells,LAT (linker for activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.,IMD52

ID gène: 27040

UniProt: O43561

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway