CD79a anticorps (AA 202-216)

N° du produit ABIN302099

L’anticorps Souris Monoclonal anti-CD79a a été validé pour FACS, IHC (p) et IHC (fro). Il convient pour détecter CD79a dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Porc, Poulet, Lapin, Cheval, Cobaye et Opossum. Il y a 5+ publications disponibles.

Aperçu rapide pour CD79a anticorps (AA 202-216) (ABIN302099)

Antigène: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Poulet, Lapin, Cheval, Cobaye, Opossum

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD79a est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HM57

Détail du produit anti-CD79a anticorps

Épitope: AA 202-216

Fonction: Anti-Hu CD79a Purified

Specificité: The antibody HM57 interacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.

Réactivité croisée (Details): Human, Porcine, Mouse, Rat, Bovine, Equine (Horse), Guinea pig, Opossum, Rabbit, Chicken, Other not determined

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Synthetic peptide corresponding to amino acids 202-216 of human CD79a

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 5 μg/mL. Intracellular staining.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL.
Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD79a (ABIN302099)

Faldyna, Samankova, Leva, Cerny, Oujezdska, Rehakova, Sinkora: "Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs." dans: Veterinary immunology and immunopathology, Vol. 119, Issue 1-2, pp. 56-62, (2007) (PubMed).

Mason, Cordell, Brown, Borst, Jones, Pulford, Jaffe, Ralfkiaer, Dallenbach, Stein: "CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples." dans: Blood, Vol. 86, Issue 4, pp. 1453-9, (1995) (PubMed).

Jones, Cordell, Beyers, Tse, Mason: "Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 12, pp. 5429-35, (1993) (PubMed).

Mason, van Noesel, Cordell, Comans-Bitter, Micklem, Tse, van Lier, van Dongen: "The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation." dans: European journal of immunology, Vol. 22, Issue 10, pp. 2753-6, (1992) (PubMed).

Mason, Cordell, Tse, van Dongen, van Noesel, Micklem, Pulford, Valensi, Comans-Bitter, Borst: "The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 11, pp. 2474-82, (1991) (PubMed).

Détails sur CD79a

Antigène: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Autre désignation: CD79a

Sujet: CD79a molecule,CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.,BCR alpha, Ig-alpha, MB-1, IGA

ID gène: 973

UniProt: P11912

Pathways: BCR Signaling