LIG4 anticorps (AA 612-911)

N° du produit ABIN3022475

Il existe 2+ publications pour ce produit. L’anticorps anti-LIG4 Polyclonal Lapin est utilisé pour la détection de LIG4 dans des échantillons de Humain. Il a été validé pour WB et IHC.

Aperçu rapide pour LIG4 anticorps (AA 612-911) (ABIN3022475)

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIG4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-LIG4 anticorps

Épitope: AA 612-911

Séquence: SKHLYIGGDD EPQEKKRKAA PKMKKVIGII EHLKAPNLTN VNKISNIFED VEFCVMSGTD SQPKPDLENR IAEFGGYIVQ NPGPDTYCVI AGSENIRVKN IILSNKHDVV KPAWLLECFK TKSFVPWQPR FMIHMCPSTK EHFAREYDCY GDSYFIDTDL NQLKEVFSGI KNSNEQTPEE MASLIADLEY RYSWDCSPLS MFRRHTVYLD SYAVINDLST KNEGTRLAIK ALELRFHGAK VVSCLAEGVS HVIIGEDHSR VADFKAFRRT FKRKFKILKE SWVTDSIDKC ELQEENQYLI

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Polyclonal Antibodies

Immunogène: Recombinant fusion protein containing a sequence corresponding to amino acids 612-911 of human DNA Ligase IV (NP_002303.2).

Isotype: IgG

Information d'application

Indications d'application: WB,1:500 - 1:2000,IHC,1:50 - 1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze / thaw cycles

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze / thaw cycles.

Références pour anticorps anti-LIG4 (ABIN3022475)

Zhang, Chang, Sun, Chen, Yang, Tang, Jing, Kang, He, Wu, Wei, Wang, Xu, Zhu, Shen, Zeng, Wang, Liu, Zhang, Mao, Jiang, Sun: "Histone H3K27 methylation modulates the dynamics of FANCD2 on chromatin to facilitate NHEJ and genome stability." dans: Journal of cell science, Vol. 131, Issue 12, (2018) (PubMed).

Li, Zhang, Chen, Guo, Zhang, Tang, Xu, Zhang, Tao, Wang, Jiang, Sun, Mao: "Impaired DNA double-strand break repair contributes to the age-associated rise of genomic instability in humans." dans: Cell death and differentiation, Vol. 23, Issue 11, pp. 1765-1777, (2017) (PubMed).

Détails sur LIG4

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Autre désignation: LIG4

Sujet: The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.,LIG4,LIG4S,Epigenetics & Nuclear Signaling,Cell Biology & Developmental Biology,Apoptosis,LIG4

Poids moléculaire: 103 kDa

ID gène: 3981

UniProt: P49917

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response