Melanoma gp100 anticorps

N° du produit ABIN3024235

Cet anticorps anti-Melanoma gp100 est un anticorps Souris Monoclonal détectant Melanoma gp100 dans IF, FACS et IHC (p). Adapté pour Humain.

Aperçu rapide pour Melanoma gp100 anticorps (ABIN3024235)

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Melanoma gp100 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: HMB45-PMEL-783

Détail du produit anti-Melanoma gp100 anticorps

Purification: Protein G affinity chromatography

Immunogène: Extract of pigmented melanoma metastases from lymph nodes (HMB45) and recombinant human protein (PMEL/783) were used as the immunogen for the gp100 antibody cocktail.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the gp100 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the gp100 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Melanoma gp100

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Autre désignation: Gp100

Sujet: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This mAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This mAb also stains Angiomyolipoma (PEComa).