Melanoma gp100 anticorps

N° du produit ABIN3024239

Cet anticorps Souris Monoclonal détecte spécifiquement Melanoma gp100 dans IF, FACS et IHC (p). Il présente une réactivité envers Humain et Cheval.

Aperçu rapide pour Melanoma gp100 anticorps (ABIN3024239)

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivité: Humain, Cheval

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Melanoma gp100 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM286

Détail du produit anti-Melanoma gp100 anticorps

Aucune reactivité croisée: Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogène: Membranes from a human melanoma metastasis were used as the immunogen for this anti-gp100 antibody.

Isotype: IgG2b kappa

Information d'application

Indications d'application: The optimal dilution of the anti-gp100 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the anti-gp100 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Melanoma gp100

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Autre désignation: Gp100

Sujet: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.