NGFR anticorps

N° du produit ABIN3024324

L’anticorps Souris Monoclonal anti-NGFR a été validé pour FACS, IF et IHC (p). Il convient pour détecter NGFR dans des échantillons de Humain et Rat.

Aperçu rapide pour NGFR anticorps (ABIN3024324)

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NGFR est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CDLA271

Détail du produit anti-NGFR anticorps

Aucune reactivité croisée: Souris, Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for the CD271 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the CD271 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD271 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur NGFR

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Autre désignation: CD271

Sujet: CD271/NGFR pays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. [UniProt]

Pathways: Signalisation NF-kappaB, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding