CD74 anticorps

N° du produit ABIN3024478

Cet anticorps anti-CD74 Monoclonal Souris (Clone SPM523) (ABIN3024478) détecte spécifiquement CD74 dans FACS, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CD74 anticorps (ABIN3024478)

Antigène: CD74 (HLA-DR-gamma (CD74))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD74 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: SPM523

Détail du produit anti-CD74 anticorps

Fonction: Anti-CD74 Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogène: SU-DHL-4 lymphoma cells were used as the immunogen for this anti-CD74 antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: The optimal dilution of the anti-CD74 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the anti-CD74 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur CD74

Antigène: CD74 (HLA-DR-gamma (CD74))

Autre désignation: CD74

Sujet: CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints