Cytochrome C anticorps (AA 93-104)

N° du produit ABIN3024593

Cet anticorps Souris Monoclonal détecte spécifiquement Cytochrome C dans WB, FACS, IF et IHC (p). Il présente une réactivité envers Humain, Rat, Souris, Cheval, Chien, Pigeon, Drosophila melanogaster et Grenouille.

Aperçu rapide pour Cytochrome C anticorps (AA 93-104) (ABIN3024593)

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivité: Humain, Rat, Souris, Cheval, Chien, Pigeon, Drosophila melanogaster, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytochrome C est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM389

Détail du produit anti-Cytochrome C anticorps

Épitope: AA 93-104

Purification: Protein G affinity chromatography

Immunogène: Amino acids 66-104 of pigeon Cytochrome C were used as the immunogen for the Cytochrome C antibody. It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C.

Isotype: IgG2b kappa

Information d'application

Indications d'application: Optimal dilution of the Cytochrome C antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Cytochrome C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Cytochrome C

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Autre désignation: Cytochrome C

Sujet: Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity