GRP94 anticorps

N° du produit ABIN3024904

Cet anticorps anti-GRP94 Monoclonal Rat (Clone SRPR90b) (ABIN3024904) détecte spécifiquement GRP94 dans WB, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour GRP94 anticorps (ABIN3024904)

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp GRP94 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SRPR90b

Détail du produit anti-GRP94 anticorps

Purification: Protein G affinity chromatography

Immunogène: Recombinant full-length human HSP90B1 protein was used as the immunogen for the GRP94 antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Titering of the GRP94 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunofluorescence: 1-2 μg/mL,Immunohistology (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the GRP94 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur GRP94

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Autre désignation: GRP94

Sujet: Heat shock protein 90  kDa beta member 1, known also as endoplasmin, gp96, grp94 and ERp99, is an HSP90 paralogue that is found in the endoplasmic reticulum. It plays critical roles in folding proteins in the secretory pathway such as Toll-like receptors and integrins. It has been implicated as an essential immune chaperone to regulate both innate and adaptive immunity. [Wiki]

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades