Keratin 5/8 anticorps

N° du produit ABIN3025013

Détail du produit anti-Keratin 5/8 anticorps

Antigène: Keratin 5/8 (KRT5/8)

Reactivité: Humain, Souris, Porc, Boeuf (Vache), Rat, Chien, Hamster, Mouton, Furet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Keratin 5/8 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aucune reactivité croisée: Poulet, Xenopus laevis

Attributs du produit: It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Purification: Protein G affinity chromatography

Immunogène: A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.

Clone: SPM268

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.

1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1 mg/mL PBS, 15 min at RT
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Cytokeratin 5/8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Keratin 5/8

Antigène: Keratin 5/8 (KRT5/8)

Autre désignation: Cytokeratin 5/8 (KRT5/8 Produits)

Sujet: It reacts with keratin 5 (58  kDa) and keratin 8 (52.5  kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.