Keratin 5/8 anticorps
Aperçu rapide pour Keratin 5/8 anticorps (ABIN3025013)
Antigène
Reactivité
Hôte
Clonalité
Conjugué
Application
Classe de qualité
Clone
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Fonction
- Cytokeratin 5/8 Antibody (azide and preservative free)
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Aucune reactivité croisée
- Poulet, Xenopus laevis
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Attributs du produit
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
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Purification
- Protein G affinity chromatography
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Immunogène
- A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.
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Isotype
- IgG1, kappa
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Indications d'application
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Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.
1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1 mg/mL PBS, 15 min at RT
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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Agent conservateur
- Azide free
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store the Cytokeratin 5/8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Keratin 5/8 (KRT5/8)
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Autre désignation
- Cytokeratin 5/8
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Sujet
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
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UniProt
- P13647
Antigène
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