Keratin 5/8 anticorps

N° du produit ABIN6941336

L’anticorps anti-Keratin 5/8 Monoclonal Souris est utilisé pour la détection de Keratin 5/8 dans des échantillons de Humain, Souris, Porc, Rat, Chien, Hamster, Boeuf (Vache), Mouton et Furet. Il a été validé pour WB, FACS, IF et IHC.

Aperçu rapide pour Keratin 5/8 anticorps (ABIN6941336)

Antigène: Keratin 5/8 (KRT5/8)

Reactivité: Humain, Souris, Porc, Rat, Chien, Hamster, Boeuf (Vache), Mouton, Furet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Keratin 5/8 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: C-50

Détail du produit anti-Keratin 5/8 anticorps

Fonction: Cytokeratin 5/8 Antibody

Specificité: It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Aucune reactivité croisée: Poulet, Xenopus laevis

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Cytoskeletal preparation from HeLa cells

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2 to 8°C. Antibody is stable for 24 months.

Date de péremption: 24 months

Détails sur Keratin 5/8

Antigène: Keratin 5/8 (KRT5/8)

Autre désignation: KRT5 & KRT8

Sujet:

CK5, DDD, EBS2, Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types, K5, Keratin complex 2, basic, Gene 5, Keratin Type II Cytoskeletal 5, Keratin-5, KRT5, KRT5A, Type II Cytoskeletal 5, Type-II Keratin Kb5. CK8, CARD2, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8

HepG2 or A431 cells. Skin or Squamous cell carcinoma.

Poids moléculaire: 58kDa (CK5), 52.5kDa (CK8)

ID gène: 3852, 3856, 433845

UniProt: P13647, P05787