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Keratin 5/8 anticorps

KRT5/8 Reactivité: Humain WB, FACS, IF, IHC (f) Hôte: Souris Monoclonal C-50 unconjugated
N° du produit ABIN6937713
  • Antigène Tous les produits Keratin 5/8 (KRT5/8)
    Keratin 5/8 (KRT5/8)
    Reactivité
    • 23
    • 19
    • 18
    • 15
    • 13
    • 13
    • 13
    • 10
    • 9
    • 5
    • 3
    • 2
    • 2
    Humain
    Hôte
    • 23
    Souris
    Clonalité
    • 23
    Monoclonal
    Conjugué
    • 10
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Keratin 5/8 est non-conjugé
    Application
    • 17
    • 15
    • 14
    • 12
    • 8
    • 7
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Specificité
    It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
    Aucune reactivité croisée
    Poulet, Xenopus laevis
    Réactivité croisée (Details)
    Human. Pig. Mouse. Rat. Hamster. Cow. Dog. Sheep. Ferret. Does not react with Chicken or Xenopus.
    Immunogène
    Cytoskeletal preparation from HeLa cells
    Clone
    C-50
    Isotype
    IgG1 kappa
  • Indications d'application

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.

    Positive_Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Agent conservateur
    Azide free
    Stock
    4 °C
    Stockage commentaire
    Store at 2 to 8°C. Antibody is stable for 24 months.
    Date de péremption
    24 months
  • Antigène
    Keratin 5/8 (KRT5/8)
    Autre désignation
    KRT5 & KRT8 (KRT5/8 Produits)
    Sujet
    CK5, DDD, EBS2, Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types, K5, Keratin complex 2, basic, Gene 5, Keratin Type II Cytoskeletal 5, Keratin-5, KRT5, KRT5A, Type II Cytoskeletal 5, Type-II Keratin Kb5. CK8, CARD2, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8,Cytokeratin 5/8
    Cellular localisation: Cytoplasmic
    Poids moléculaire
    58kDa (CK5), 52.5kDa (CK8)
    ID gène
    3852, 3856, 433845
    UniProt
    P13647, P05787
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