NGFR anticorps (Extracellular)

N° du produit ABIN3025050

L’anticorps anti-NGFR Monoclonal Souris est utilisé pour la détection de NGFR dans des échantillons de Humain. Il a été validé pour IHC (p).

Aperçu rapide pour NGFR anticorps (Extracellular) (ABIN3025050)

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NGFR est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM299

Détail du produit anti-NGFR anticorps

Épitope: AA 1-160, Extracellular

Fonction: NGF Receptor Antibody

Aucune reactivité croisée: Souris, Rat (Rattus)

Attributs du produit: It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.

Purification: Protein G affinity chromatography

Immunogène: NGFR from A875 melanoma cells was used as the immunogen for the NGF Receptor antibody. Its epitope is within amino acids 1-160 of the extracellular domain of NGFR/NTR.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the NGF Receptor antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the NGF Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur NGFR

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Autre désignation: NGF Receptor

Sujet: It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.

UniProt: P08138

Pathways: Signalisation NF-kappaB, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding