MITF anticorps (N-Term)

N° du produit ABIN3025233

L’anticorps Souris Monoclonal anti-MITF a été validé pour WB, IF, FACS et IHC (p). Il convient pour détecter MITF dans des échantillons de Humain.

Aperçu rapide pour MITF anticorps (N-Term) (ABIN3025233)

Antigène: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MITF est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: D5

Détail du produit anti-MITF anticorps

Épitope: N-Term

Aucune reactivité croisée: Souris, Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogène: An N-terminus fragment of human Mi protein was used as the immunogen for the MITF antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the MITF antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the MITF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur MITF

Antigène: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Autre désignation: MITF

Sujet: MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.

Pathways: Chromatin Binding