PODXL anticorps (Extracellular Domain)
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- Antigène Voir toutes PODXL Anticorps
- PODXL (Podocalyxin-Like (PODXL))
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Épitope
- Extracellular Domain
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Reactivité
- Humain, Rat, Lapin
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp PODXL est non-conjugé
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogène
- A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody.
- Clone
- 3D3
- Isotype
- IgG1
- Top Product
- Discover our top product PODXL Anticorps primaire
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- Indications d'application
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Podocalyxin antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IP: 1-2 μg/500 μg protein,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Agent conservateur
- Azide free
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Antigène
- PODXL (Podocalyxin-Like (PODXL))
- Autre désignation
- Podocalyxin (PODXL Produits)
- Synonymes
- anticorps Gp200, anticorps PC, anticorps PCLP, anticorps PCLP-1, anticorps PCB, anticorps AW121214, anticorps Ly102, anticorps Pclp1, anticorps podocalyxin, anticorps MEP21, anticorps PODXL, anticorps Gp135, anticorps PCLP1, anticorps cPCLP1, anticorps podxl, anticorps fc18d02, anticorps wu:fc18d02, anticorps Pc, anticorps Pcb, anticorps Podxl, anticorps podocalyxin like, anticorps pyruvate carboxylase, anticorps podocalyxin-like, anticorps PODXL, anticorps PC, anticorps Podxl, anticorps podxl, anticorps Pcx
- Sujet
- Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
- ID gène
- 5420
- Pathways
- Tube Formation
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