S100A9 anticorps

N° du produit ABIN3025524

L’anticorps Souris Monoclonal anti-S100A9 a été validé pour IF, FACS et IHC (p). Il convient pour détecter S100A9 dans des échantillons de Humain.

Aperçu rapide pour S100A9 anticorps (ABIN3025524)

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp S100A9 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: S100A9-1011

Détail du produit anti-S100A9 anticorps

Purification: PEG precipitation

Immunogène: Recombinant human protein was used as the immunogen for the S100A9 antibody.

Isotype: IgM kappa

Information d'application

Indications d'application: Optimal dilution of the S100A9 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Formalin-fixed): 0.5-1 μg/mL for 30 min at RT (1),Not suitable for frozen tissues

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the S100A9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur S100A9

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Autre désignation: S100A9

Sujet: Recognizes a 14  kDa protein, identified as S100A9 (also known as Calgranulin B or MRP14), expressed by granulocytes, monocytes and by tissue macrophages. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This mAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins